Which technique is used to separate protein fragments based on size?polyacrylamide gel electrophoresis Southern blot agarose gel electrophoresis polymerase chain reaction
A. Polyacrylamide gel electrophoresis (PAGE)
Protein fragments of different size can be separated by gel electrophoresis method. Different proteins are made up of different number of amino acids and so the chain length vary i.e. the size of different proteins may vary. Also different proteins may have different charges due to difference in the type of amino acids present in them.
Generally for separating the protein fragments according to their size polyacrylamide gel is used. Foe this proteins are first given the heat treatment and treated with SDS for unfolding the protein. SDS is a detergent that imparts negative charge to protein. During electrophoresis, under the electric field the separation would be only due to size but not charge as all the proteins are treated with SDS and thus have same negative charge. This method is called SDS-PAGE. Under the electric field, small protein fragments will move fast through the pores compared to large fragments leading to their separation.
If two proteins are of same size then to separate them wither agarose gel electrophoresis is used or isoelectric focusing.
A researcher is studying gene expression in different types of cells. For a gene of interest, he notes its expression no longer appears to be regulated—it occurs in all cells, rather than in just a few cell types. The gene’s corresponding transcript and protein, however, appear normal. This may be the result of a mutation in which of the following regions associated with the gene? (5 POINTS)Silencer
9What role does the MutL protein play in DNA repair? (5 POINTS)
It performs a proofreading function, similar to DNA polymerase, and removes incorrect bases from the template DNA strand.
It recognizes and binds to a pair of "mismatched" nucleotides, preventing their translation.
It recognizes the newly-synthesized DNA strand (e.g., by detecting nicks), ensuring only it—and not the template strand—is repaired.
It helps to link together regions of DNA, after an error has been removed via exonuclease.
10If a mutation occurs that affects the large subunit of a ribosome, rendering it inactive, how would this affect translation? (5 POINTS)
The small subunit of the ribosome would fail to associate with a mRNA.
Methionine tRNA would not recognize the start codon of a mRNA.
New amino acids would not be added after the initial methionine, so translation would be effectively inhibited.
Arginine tRNA, rather than methionine tRNA, would associate with a mRNA’s stop codon.
8) The region associated with the gene is : ( A ) Silencer
9) The role played by Mutl protein in DNA repair is : ( C ) It recognizes the newly-synthesized DNA strand (e.g., by detecting nicks), ensuring only it—and not the template strand—is repaired.
10) The effect on translation is : ( C ) New amino acids would not be added after the initial methionine, so translation would be effectively inhibited.
Function of the silencer
The silencer is the region of the DNA that regulates the expression of DNA in RNA and protein, since the mutation cannot regulate the expression of genes the region associated with this is the silencer.
Mutl proteins helps in identifying template DNA strand and newly synthesized DNA strand. they are also used in the treatment of methyl mismatch. also
Translation is inhibited when a large unit of ribosomes is affected by a mutation.
Hence we can conclude that The region associated with the gene is : Silencer. The role played by Mutl protein in DNA repair is :It recognizes the newly-synthesized DNA strand (e.g., by detecting nicks), ensuring only it—and not the template strand—is repaired.The effect on translation is New amino acids would not be added after the initial methionine, so translation would be effectively inhibited.
9- It recognizes the newly-synthesized DNA strand (e.g., by detecting nicks), ensuring only it—and not the template strand—is repaired
10- New amino acids would not be added after the initial methionine, so translation would be effectively inhibited.
As the question is, mutation is unable to regulate gene expression and occurs in all cells. Thus, silencer are the DNA control region and regulate the expression of DNA into RNA and protein. Hence, option a is correct.
This protein is used to differentiate between tempelate DNA strand and newerly synthesized DNA strand to rectify the foundation incorporated during DNA replication; it helps to identify new synthesizes of DNA. MutL Proteines are a proteins used to treat a methyl mismatch repair system. Hence, option c is correct.
If the large unit of ribosome is affected by a mutation, new amino acid is not introduced after inhibition of the original methionine and translation. Hence, option c is correct.
Building material examination
Construction materials testing (CMT) is a vital process that helps builders and site owners identify potential problems before committing resources to the project. Testing is also essential for keeping the structure in line with applicable legal requirements, including occupational safety and environmental regulations.
1. Which is NOT an energy type? A. non-renewable B. valuable C. renewable
non renewable: oil
hope i could help
valuable is not an energy type
A particular function performed by an arrangement of similar cells is characteristic of .
The answer would be tissues.
What is the relationship between an individual named pinus palustris and an individual named pinus elliottii?
In scientific names, the genus is named first, then the species. For example, humans are Homo sapiens, where we belong in genus "homo" and our species is called "sapiens." Hope that hint is helpful. :)